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  • Featured Product
  • KD/KO Validated

BCAS2 Polyclonal antibody

BCAS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 10414-1-AP

Synonyms

Spliceosome-associated protein SPF 27, Pre-mRNA-splicing factor SPF27, Pre mRNA splicing factor SPF27, DAM1, Breast carcinoma-amplified sequence 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCAS2 Polyclonal antibody (10414-1-AP)

    WB analysis using 10414-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BCAS2 Polyclonal antibody (10414-1-AP)

    WB analysis of HeLa using 10414-1-AP

    WB result of BCAS2 antibody (10414-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAS2 transfected HeLa cells.

  • Western Blot (WB) analysis of human brain tissue using BCAS2 Polyclonal antibody (10414-1-AP)

    WB analysis of human brain using 10414-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using BCAS2 Polyclonal antibody (10414-1-AP)

    WB analysis of K-562 using 10414-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COS-7 cells using BCAS2 Polyclonal antibody (10414-1-AP)

    WB analysis of COS-7 using 10414-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using BCAS2 Polyclonal antibody (10414-1-AP)

    WB analysis of mouse brain using 10414-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using BCAS2 Polyclonal antibody (10414-1-AP)

    WB analysis of mouse liver using 10414-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of hESC cells using BCAS2 Polyclonal antibody (10414-1-AP)

    WB analysis of hESC using 10414-1-AP

    hESC cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BCAS2 Polyclonal antibody (10414-1-AP)

    WB analysis of HeLa using 10414-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using BCAS2 Polyclonal antibody (10414-1-AP)

    WB analysis of SH-SY5Y using 10414-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using BCAS2 Polyclonal antibody (10414-1-AP)

    IP experiment of mouse brain using 10414-1-AP

    IP result of anti-BCAS2 (IP:10414-1-AP, 3ug; Detection:10414-1-AP 1:1000) with mouse brain tissue lysate 6500ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BCAS2 Polyclonal antibody (10414-1-AP)

    IHC staining of human prostate cancer using 10414-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10414-1-AP (BCAS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BCAS2 Polyclonal antibody (10414-1-AP)

    IHC staining of human breast cancer using 10414-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BCAS2 Polyclonal antibody (10414-1-AP)

    IHC staining of human breast cancer using 10414-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BCAS2 Polyclonal antibody (10414-1-AP)

    IHC staining of human colon cancer using 10414-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:100 (under 25x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BCAS2 Polyclonal antibody (10414-1-AP)

    IHC staining of human prostate cancer using 10414-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10414-1-AP (BCAS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BCAS2 Polyclonal antibody (10414-1-AP)

    IF Staining of HepG2 using 10414-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BCAS2 Polyclonal antibody (10414-1-AP)

    IF Staining of MCF-7 using 10414-1-AP

    Immunofluorescent analysis of MCF-7 cells using 10414-1-AP (BCAS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse liver tissue, hESC cells, human brain tissue, K-562 cells, COS-7 cells, SH-SY5Y cells, PC-12 cells, C6 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10414-1-AP targets BCAS2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAS2 fusion protein Ag0669
Full Name breast carcinoma amplified sequence 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26-32 kDa
GenBank Accession NumberBC005285
Gene Symbol BCAS2
Gene ID (NCBI) 10286
RRIDAB_2063400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCAS2 associates with the spliceosome and implicates in mRNA splicing

Protocols

Product Specific Protocols
WB protocol for BCAS2 antibody 10414-1-APDownload protocol
IHC protocol for BCAS2 antibody 10414-1-APDownload protocol
IF protocol for BCAS2 antibody 10414-1-APDownload protocol
IP protocol for BCAS2 antibody 10414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis.

Authors - Wenbo Liu
View Article
WB

Int J Biochem Cell Biol

BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination.

Authors - Shengjie Liao
View Article
mouseWB,IF

Stem Cell Res Ther

Breast carcinoma-amplified sequence 2 regulates adult neurogenesis via β-catenin.

Authors - Hsin-Hsiung Chen
  • KD Validated
View Article
humanIHC, WB

Br J Cancer

BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability.

Authors - P-C Kuo
  • KD Validated
View Article
mouseWB,IF

FASEB J

BCAS2 is involved in alternative splicing and mouse oocyte development.

Authors - Jiaqi Zhang
  • KO Validated
View Article
mouseWB,RIP

Endocrinology

BCAS2 participates in insulin synthesis and secretion via mRNA alternative splicing in mice

Authors - Xuexue Chen
View Article