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BCAS1 Polyclonal antibody

BCAS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25776-1-AP

Synonyms

NABC1, Breast carcinoma-amplified sequence 1

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using BCAS1 Polyclonal antibody (25776-1-AP)

    WB analysis of NIH/3T3 using 25776-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25776-1-AP (BCAS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BCAS1 Polyclonal antibody (25776-1-AP)

    WB analysis of MCF-7 using 25776-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25776-1-AP (BCAS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using BCAS1 Polyclonal antibody (25776-1-AP)

    IHC staining of human stomach using 25776-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25776-1-AP (BCAS1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BCAS1 Polyclonal antibody (25776-1-AP)

    IF Staining of MCF-7 using 25776-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using BCAS1 antibody (25776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inNIH/3T3 cells, MCF-7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25776-1-AP targets BCAS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAS1 fusion protein Ag22760
Full Name breast carcinoma amplified sequence 1
Calculated Molecular Weight 584 aa, 62 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC126348
Gene Symbol BCAS1
Gene ID (NCBI) 8537
RRIDAB_2880235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BCAS1 antibody 25776-1-APDownload protocol
IHC protocol for BCAS1 antibody 25776-1-APDownload protocol
IF protocol for BCAS1 antibody 25776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols