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BCAR3 Polyclonal antibody

BCAR3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13628-1-AP

Synonyms

BCAR3, KIAA0554, Novel SH2 containing protein 2, NSP2, SH2D3B

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using BCAR3 Polyclonal antibody (13628-1-AP)

    WB analysis of K-562 using 13628-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13628-1-AP (BCAR3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat adrenal gland tissue using BCAR3 Polyclonal antibody (13628-1-AP)

    IHC staining of rat adrenal gland using 13628-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 13628-1-AP (BCAR3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inrat adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13628-1-AP targets BCAR3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAR3 fusion protein Ag4546
Full Name breast cancer anti-estrogen resistance 3
Calculated Molecular Weight 825 aa, 93 kDa
Observed Molecular Weight 92-105 kDa
GenBank Accession NumberBC039895
Gene Symbol BCAR3
Gene ID (NCBI) 8412
RRIDAB_2877962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BCAR3 antibody 13628-1-APDownload protocol
IHC protocol for BCAR3 antibody 13628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Int J Nanomedicine

Localized Administration of Bcar3 siRNA via Nano-Self-Assembly to Treat Idiopathic Pulmonary Fibrosis by Disrupting Macrophage-Fibroblast Crosstalk

Authors - Chenxi Zeng
View Article