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BBS5 Polyclonal antibody

BBS5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA, IF

Conjugate

Unconjugated

Cat no : 14569-1-AP

Synonyms

Bardet Biedl syndrome 5, BBS5



Tested Applications

Positive WB detected inmouse retina tissue, mouse testis tissue, human testis tissue, rat testis tissue, mouse eye tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, mouse testis tissue, mouse kidney tissue, human stomach tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14569-1-AP targets BBS5 in WB, IHC, IP, CoIP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS5 fusion protein Ag6153
Full Name Bardet-Biedl syndrome 5
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC044593
Gene Symbol BBS5
Gene ID (NCBI) 129880
RRIDAB_10597551
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.

Protocols

Product Specific Protocols
WB protocol for BBS5 antibody 14569-1-APDownload protocol
IHC protocol for BBS5 antibody 14569-1-APDownload protocol
IP protocol for BBS5 antibody 14569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR
humanIF

Cell

The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.

Authors - Jin Hua H
humanWB

Nat Cell Biol

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Authors - Malavika Raman
mouseWB,IF

Dev Cell

IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.

Authors - Thibaut Eguether
mouseIF

Dev Cell

The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3.

Authors - Gerald M Liew
ratIF

Nat Struct Mol Biol

Structural basis for membrane targeting of the BBSome by ARL6.

Authors - André Mourão