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  • Featured Product
  • KD/KO Validated

BAG5 Polyclonal antibody

BAG5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26628-1-AP

Synonyms

KIAA0873, Bcl-2-associated athanogene 5, BAG-5, BAG 5

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using BAG5 Polyclonal antibody (26628-1-AP)

    WB analysis of MCF-7 using 26628-1-AP

    WB result of BAG5 antibody (26628-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG5 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using BAG5 Polyclonal antibody (26628-1-AP)

    WB analysis using 26628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26628-1-AP (BAG5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using BAG5 Polyclonal antibody (26628-1-AP)

    IHC staining of human thyroid cancer using 26628-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26628-1-AP (BAG5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using BAG5 Polyclonal antibody (26628-1-AP)

    IHC staining of human thyroid cancer using 26628-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26628-1-AP (BAG5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BAG5 Polyclonal antibody (26628-1-AP)

    IF Staining of MCF-7 using 26628-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BAG5 antibody (26628-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inMCF-7 cells, SH-SY5Y cells, mouse brain, rat brain
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26628-1-AP targets BAG5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAG5 fusion protein Ag24320
Full Name BCL2-associated athanogene 5
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 48-51 kDa
GenBank Accession NumberBC044216
Gene Symbol BAG5
Gene ID (NCBI) 9529
RRIDAB_2880578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BAG5 antibody 26628-1-APDownload protocol
IHC protocol for BAG5 antibody 26628-1-APDownload protocol
IF protocol for BAG5 antibody 26628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Discov

Neuromodulation of BAG co-chaperones by HIV-1 viral proteins and H2O2: implications for HIV-associated neurological disorders.

Authors - Michael R Duggan
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

WEI (Verified Customer) (03-03-2022)

It have three bands, one band is missing in the KO tissue.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1: 3000
  • Cell Tissue Type: Mouse Heart