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BAAT Polyclonal antibody

BAAT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15990-1-AP

Synonyms

BAAT, BACAT, BAT, Glycine N choloyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using BAAT Polyclonal antibody (15990-1-AP)

    WB analysis of human liver using 15990-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15990-1-AP (BAAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using BAAT Polyclonal antibody (15990-1-AP)

    IHC staining of human hepatocirrhosis using 15990-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15990-1-AP (BAAT Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using BAAT Polyclonal antibody (15990-1-AP)

    IHC staining of human hepatocirrhosis using 15990-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15990-1-AP (BAAT Antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BAAT Polyclonal antibody (15990-1-AP)

    IF Staining of HepG2 using 15990-1-AP

    Immunofluorescent analysis of HepG2 cells using 15990-1-AP (BAAT antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15990-1-AP targets BAAT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAAT fusion protein Ag8860
Full Name bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase)
Calculated Molecular Weight 418 aa, 46 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC009567
Gene Symbol BAAT
Gene ID (NCBI) 570
RRIDAB_2061361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAAT is bile acid-CoA:amino acid N-acyltransferase which involved in in bile acid metabolism. BAAT acts as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids.

Protocols

Product Specific Protocols
WB protocol for BAAT antibody 15990-1-APDownload protocol
IHC protocol for BAAT antibody 15990-1-APDownload protocol
IF protocol for BAAT antibody 15990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Toxicol In Vitro

Toxicity and intracellular accumulation of bile acids in sandwich-cultured rat hepatocytes: Role of glycine conjugates.

Authors - Chatterjee Sagnik S
View Article