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BAALC Polyclonal antibody

BAALC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24997-1-AP

Synonyms

BAALC

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using BAALC Polyclonal antibody (24997-1-AP)

    WB analysis of HEK-293 using 24997-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24997-1-AP (BAALC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using BAALC Polyclonal antibody (24997-1-AP)

    WB analysis of A375 using 24997-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 24997-1-AP (BAALC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using BAALC Polyclonal antibody (24997-1-AP)

    WB analysis of mouse brain using 24997-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24997-1-AP (BAALC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using BAALC Polyclonal antibody (24997-1-AP)

    IP experiment of mouse brain using 24997-1-AP

    IP result of anti-BAALC (IP:24997-1-AP, 4ug; Detection:24997-1-AP 1:300) with mouse brain tissue lysate 3000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BAALC Polyclonal antibody (24997-1-AP)

    IHC staining of mouse brain using 24997-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24997-1-AP (BAALC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using BAALC Polyclonal antibody (24997-1-AP)

    IHC staining of mouse brain using 24997-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24997-1-AP (BAALC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BAALC Polyclonal antibody (24997-1-AP)

    IF Staining of HEK-293 using 24997-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24997-1-AP (BAALC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, A375 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 24997-1-AP is BAALC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAALC fusion protein Ag21850
Full Name brain and acute leukemia, cytoplasmic
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19-25 kDa
GenBank Accession NumberBC011517
Gene Symbol BAALC
Gene ID (NCBI) 79870
RRIDAB_2879837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Brain and acute leukemia cytoplasmic protein, also named as BAALC, is a 180 amino acid protein, which interacts with CAMK2A. BAALC localizes in the cytoplasm and membrane. BAALC is expressed in neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. BAALC may play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A.

Protocols

Product Specific Protocols
WB protocol for BAALC antibody 24997-1-APDownload protocol
IHC protocol for BAALC antibody 24997-1-APDownload protocol
IF protocol for BAALC antibody 24997-1-APDownload protocol
IP protocol for BAALC antibody 24997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Chin Med J (Engl)

Expression and prognostic value of brain and acute leukemia, cytoplasmic in meningiomas.

Authors - Yong-Tao He
View Article