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B4GALT3 Polyclonal antibody

B4GALT3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11041-1-AP

Synonyms

beta4Gal T3, Beta-1,4-GalTase 3, Beta-1,4-galactosyltransferase 3, Beta 1,4 GalTase 3, b4Gal-T3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using B4GALT3 Polyclonal antibody (11041-1-AP)

    WB analysis using 11041-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11041-1-AP (B4GALT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using B4GALT3 Polyclonal antibody (11041-1-AP)

    IHC staining of human liver cancer using 11041-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using B4GALT3 Polyclonal antibody (11041-1-AP)

    IHC staining of human liver cancer using 11041-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using B4GALT3 Polyclonal antibody (11041-1-AP)

    IHC staining of human breast cancer using 11041-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using B4GALT3 Polyclonal antibody (11041-1-AP)

    IHC staining of human breast cancer using 11041-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using B4GALT3 Polyclonal antibody (11041-1-AP)

    IF Staining of A431 using 11041-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 11041-1-AP (B4GALT3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:300
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11041-1-AP targets B4GALT3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALT3 fusion protein Ag1487
Full Name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
Calculated Molecular Weight 44 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC009985
Gene Symbol B4GALT3
Gene ID (NCBI) 8703
RRIDAB_2290072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B4GALT3 is an enzyme responsible for the generation of poly-N-acetyllactosamine. The B4GALTs transfer galactose from UDP-Gal to N-acetylglucosamine (GlcNAc)-terminated oligosaccharides on N-glycan, O-glycan, or glycolipid to form N-acetyllactosamin (PMID: 23444218). B4GALT3 has been found to regulate cancer cell invasion (PMID: 25659296). B4GALT3 knockdown increased cell migration, invasion and the activation of β1 integrin and its downstream signaling (PMID: 24403309).

Protocols

Product Specific Protocols
WB protocol for B4GALT3 antibody 11041-1-APDownload protocol
IHC protocol for B4GALT3 antibody 11041-1-APDownload protocol
IF protocol for B4GALT3 antibody 11041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Int J Mol Sci

Mutations in GDAP1 Influence Structure and Function of the Trans-Golgi Network.

Authors - Katarzyna Binięda
View Article
humanWB

Aging (Albany NY)

Circular RNA circUBXN7 represses cell growth and invasion by sponging miR-1247-3p to enhance B4GALT3 expression in bladder cancer.

Authors - Hongwei Liu
View Article
humanWB

Nat Commun

Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2

Authors - Karsten Nalbach
View Article
humanWB

Aging (Albany NY)

Development and validation of glycosyltransferase related-gene for the diagnosis and prognosis of head and neck squamous cell carcinoma

Authors - Miao He
View Article