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B4GALT2 Polyclonal antibody

B4GALT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20330-1-AP

Synonyms

B4Gal T2, B4Gal T3, B4GALT2, Beta 1,4 GalTase 2, beta4Gal T2

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using B4GALT2 Polyclonal antibody (20330-1-AP)

    WB analysis of PC-3 using 20330-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse large intestine tissue using B4GALT2 Polyclonal antibody (20330-1-AP)

    WB analysis of mouse large intestine using 20330-1-AP

    mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using B4GALT2 Polyclonal antibody (20330-1-AP)

    WB analysis of mouse brain using 20330-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using B4GALT2 Polyclonal antibody (20330-1-AP)

    WB analysis of mouse ovary using 20330-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using B4GALT2 Polyclonal antibody (20330-1-AP)

    WB analysis of mouse skeletal muscle using 20330-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B4GALT2 Polyclonal antibody (20330-1-AP)

    IHC staining of human colon cancer using 20330-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 20330-1-AP (B4GALT2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B4GALT2 Polyclonal antibody (20330-1-AP)

    IHC staining of human colon cancer using 20330-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 20330-1-AP (B4GALT2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B4GALT2 Polyclonal antibody (20330-1-AP)

    IHC staining of human colon cancer using 20330-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 20330-1-AP (B4GALT2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B4GALT2 Polyclonal antibody (20330-1-AP)

    IHC staining of human colon cancer using 20330-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 20330-1-AP (B4GALT2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using B4GALT2 Polyclonal antibody (20330-1-AP)

    IHC staining of human ovary using 20330-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20330-1-AP (B4GALT2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using B4GALT2 Polyclonal antibody (20330-1-AP)

    IHC staining of human ovary using 20330-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20330-1-AP (B4GALT2 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inPC-3 cells, mouse brain tissue, mouse large intestine tissue, mouse ovary tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20330-1-AP is B4GALT2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B4GALT2 fusion protein Ag14046
Full Name UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2
Calculated Molecular Weight 372 aa, 42 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC002431
Gene Symbol B4GALT2
Gene ID (NCBI) 8704
RRIDAB_10693615
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for B4GALT2 antibody 20330-1-APDownload protocol
IHC protocol for B4GALT2 antibody 20330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Genet

Identification of a Novel Glycosyltransferase Prognostic Signature in Hepatocellular Carcinoma Based on LASSO Algorithm.

Authors - Zhiyang Zhou
View Article