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B3GALT6 Polyclonal antibody

B3GALT6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55049-1-AP

Synonyms

B3GALT6, Beta 1,3 GalTase 6, Beta3Gal T6, beta3GalT6, GAG GalTII, Galactosyltransferase II

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using B3GALT6 Polyclonal antibody (55049-1-AP)

    WB analysis of mouse brain using 55049-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55049-1-AP (B3GALT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using B3GALT6 Polyclonal antibody (55049-1-AP)

    WB analysis of mouse liver using 55049-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 55049-1-AP (B3GALT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using B3GALT6 Polyclonal antibody (55049-1-AP)

    IHC staining of human liver cancer using 55049-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using B3GALT6 Polyclonal antibody (55049-1-AP)

    IHC staining of human liver cancer using 55049-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using B3GALT6 Polyclonal antibody (55049-1-AP)

    IHC staining of human liver using 55049-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using B3GALT6 Polyclonal antibody (55049-1-AP)

    IHC staining of human liver using 55049-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using B3GALT6 Polyclonal antibody (55049-1-AP)

    IHC staining of human liver cancer using 55049-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using B3GALT6 Polyclonal antibody (55049-1-AP)

    IHC staining of human liver cancer using 55049-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 55049-1-AP (B3GALT6 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55049-1-AP targets B3GALT6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberNM_080605
Gene Symbol B3GALT6
Gene ID (NCBI) 126792
RRIDAB_10888634
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B3GALT6 belongs to the glycosyltransferase 31 family. It transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. B3GALT6 has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. It has no activity towards substrates with terminal glucosamine or galactosamine residues. The antibody is specific to B3GALT6.

Protocols

Product Specific Protocols
WB protocol for B3GALT6 antibody 55049-1-APDownload protocol
IHC protocol for B3GALT6 antibody 55049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Microbiol

Sulfated glycosaminoglycans are host epithelial cell targets of the Candida albicans toxin candidalysin

Authors - Jianfeng Lin
View Article