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  • Featured Product
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Annexin V Monoclonal antibody

Annexin V Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E6A8

Cat no : 66245-1-Ig

Synonyms

Anchorin CII, Annexin 5, annexin A5, Annexin V, ANX5, ANXA5, Calphobindin I, CBP I, Endonexin II, ENX2, Lipocortin V, PAP I, PP4, Thromboplastin inhibitor, VAC alpha, Vascular anticoagulant alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin V Monoclonal antibody (66245-1-Ig)

    WB analysis using 66245-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Annexin V Monoclonal antibody (66245-1-Ig)

    WB analysis of HeLa using 66245-1-Ig

    WB result of Annexin V antibody (66245-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin V transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using Annexin V Monoclonal antibody (66245-1-Ig)

    WB analysis using 66245-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Annexin V Monoclonal antibody (66245-1-Ig)

    WB analysis of HEK-293 using 66245-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Annexin V Monoclonal antibody (66245-1-Ig)

    IHC staining of human malignant melanoma using 66245-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Annexin V Monoclonal antibody (66245-1-Ig)

    IHC staining of human malignant melanoma using 66245-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Annexin V Monoclonal antibody (66245-1-Ig)

    IHC staining of human spleen using 66245-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Annexin V Monoclonal antibody (66245-1-Ig)

    IHC staining of human spleen using 66245-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Annexin V Monoclonal antibody (66245-1-Ig)

    IHC staining of human colon using 66245-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Annexin V Monoclonal antibody (66245-1-Ig)

    IHC staining of human colon using 66245-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin V Monoclonal antibody (66245-1-Ig)

    IF Staining of HeLa using 66245-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin V antibody (66245-1-Ig, Clone: 1E6A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Annexin V Monoclonal antibody (66245-1-Ig)

    IF Staining of HeLa using 66245-1-Ig

    Immunofluorescent analysis of HeLa cells using 66245-1-Ig (Annexin V antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human urine exosomes tissue, Raw 264.7 cells, NIH/3T3 cells, K-562 cells, HepG2 cells
Positive IHC detected inhuman malignant melanoma tissue, human colon tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:15000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

66245-1-Ig targets Annexin V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Annexin V fusion protein Ag1538
Full Name annexin A5
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC001429
Gene Symbol Annexin V
Gene ID (NCBI) 308
RRIDAB_2881634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Protocols

Product Specific Protocols
WB protocol for Annexin V antibody 66245-1-IgDownload protocol
IHC protocol for Annexin V antibody 66245-1-IgDownload protocol
IF protocol for Annexin V antibody 66245-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

PLoS Pathog

IL-17RA promotes pathologic epithelial inflammation in a mouse model of upper respiratory influenza infection

Authors - Zahrasadat Navaeiseddighi
View Article