Acetyl-Tubulin (Lys40) Monoclonal antibody
Acetyl-Tubulin (Lys40) Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, canine and More (4)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
7E5H8
Cat no : 66200-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat testis tissue, NCCIT cells, Neuro-2a cells, L02 cells, pig cerebellum tissue, rat brain tissue, mouse ovary tissue, HEK-293 cells, mouse testis |
Positive IHC detected in | mouse ovary tissue, human gliomas tissue, rat brain tissue, mouse brain tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | hTERT-RPE1 cells, MDCK cells |
Positive ELISA detected in | Recombinant protein |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 49 publications below |
IHC | See 3 publications below |
IF | See 47 publications below |
Product Information
66200-1-Ig targets Acetyl-Tubulin (Lys40) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.
Tested Reactivity | human, mouse, rat, pig, canine |
Cited Reactivity | human, mouse, rat, pig, rabbit, canine, zebrafish, bovine, drosophila |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | tubulin, alpha 1a |
Calculated Molecular Weight | 52 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | NM_006009 |
Gene Symbol | Alpha Tubulin |
Gene ID (NCBI) | 7846 |
RRID | AB_2722562 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
What is the molecular weight of acetyl-α-tubulin?
The molecular weight of acetylated tubulin is 52 kD.
Where does the acetylation of Lys 40 occur?
This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).
How can acetylation be reversed?
Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).
What is the function of acetylation?
Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).
Is acetylation of α-tubulin strictly associated with stable microtubules?
Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).
Is ac-tubulin found only in cilia?
Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).
What are the cellular effects of tubulin acetylation?
Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).
Protocols
Product Specific Protocols | |
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WB protocol for Acetyl-Tubulin (Lys40) antibody 66200-1-Ig | Download protocol |
IHC protocol for Acetyl-Tubulin (Lys40) antibody 66200-1-Ig | Download protocol |
IF protocol for Acetyl-Tubulin (Lys40) antibody 66200-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Stem Cell Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury. | ||
Adv Sci (Weinh) DNALI1 Promotes Neurodegeneration after Traumatic Brain Injury via Inhibition of Autophagosome-Lysosome Fusion | ||
J Clin Invest Nasal ciliated cells are primary targets for SARS-CoV-2 replication in early stage of COVID-19. | ||
Biomaterials A virus-induced kidney disease model based on organ-on-a-chip: Pathogenesis exploration of virus-related renal dysfunctions. | ||
Dev Cell HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Mi (Verified Customer) (05-29-2023) | Excellent antibody, it works pretty well in human white adipocytes!
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FH Sonia (Verified Customer) (04-08-2022) | Very good specificity and sensitivity. I used for IHC in different paraffin-embedded cell blocks for different cell lines, previously analyzed by WB.
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