Acetyl-Tubulin (Lys40) Monoclonal antibody

Acetyl-Tubulin (Lys40) Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, canine and More (4)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

7E5H8

Cat no : 66200-1-Ig

Synonyms

acetylated alpha tubulin, TUBA1A, Tubulin alpha 1A chain, 7E5H8, Detyrosinated tubulin alpha-1A chain



Tested Applications

Positive WB detected inrat testis tissue, NCCIT cells, Neuro-2a cells, L02 cells, pig cerebellum tissue, rat brain tissue, mouse ovary tissue, HEK-293 cells, mouse testis
Positive IHC detected inmouse ovary tissue, human gliomas tissue, rat brain tissue, mouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells
Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66200-1-Ig targets Acetyl-Tubulin (Lys40) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.

Tested Reactivity human, mouse, rat, pig, canine
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, zebrafish, bovine, drosophila
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, alpha 1a
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberNM_006009
Gene Symbol Alpha Tubulin
Gene ID (NCBI) 7846
RRIDAB_2722562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the molecular weight of acetyl-α-tubulin?

The molecular weight of acetylated tubulin is 52 kD.

 

Where does the acetylation of Lys 40 occur?

This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274).

 

How can acetylation be reversed?

Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337).

 

What is the function of acetylation?

Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043).

 

Is acetylation of α-tubulin strictly associated with stable microtubules?

Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043).

 

Is ac-tubulin found only in cilia?

Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247).

 

What are the cellular effects of tubulin acetylation?

Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337).


Protocols

Product Specific Protocols
WB protocol for Acetyl-Tubulin (Lys40) antibody 66200-1-IgDownload protocol
IHC protocol for Acetyl-Tubulin (Lys40) antibody 66200-1-IgDownload protocol
IF protocol for Acetyl-Tubulin (Lys40) antibody 66200-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Stem Cell

Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury.

Authors - Aleksandra Tata
mouseIF

Adv Sci (Weinh)

DNALI1 Promotes Neurodegeneration after Traumatic Brain Injury via Inhibition of Autophagosome-Lysosome Fusion

Authors - Xulong Ding
humanIF

J Clin Invest

Nasal ciliated cells are primary targets for SARS-CoV-2 replication in early stage of COVID-19.

Authors - Ji Hoon Ahn
mouseWB

Aging Cell

SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.

Authors - Yingying Zhao
WB

Biomaterials

A virus-induced kidney disease model based on organ-on-a-chip: Pathogenesis exploration of virus-related renal dysfunctions.

Authors - Ji Wang
DrosophilaWB

Dev Cell

HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.

Authors - Yan Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Mi (Verified Customer) (05-29-2023)

Excellent antibody, it works pretty well in human white adipocytes!

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: adipocytes
FH

Sonia (Verified Customer) (04-08-2022)

Very good specificity and sensitivity. I used for IHC in different paraffin-embedded cell blocks for different cell lines, previously analyzed by WB.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:1000