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CARNS1 Polyclonal antibody

CARNS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25725-1-AP

Synonyms

ATP grasp domain containing 1, ATPGD1, Carnosine synthase 1, CARNS1, KIAA1394

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CARNS1 Polyclonal antibody (25725-1-AP)

    WB analysis using 25725-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25725-1-AP (CARNS1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CARNS1 Polyclonal antibody (25725-1-AP)

    IHC staining of mouse liver using 25725-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25725-1-AP (CARNS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CARNS1 Polyclonal antibody (25725-1-AP)

    IHC staining of mouse liver using 25725-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25725-1-AP (CARNS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

25725-1-AP targets CARNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARNS1 fusion protein Ag22812
Full Name ATP-grasp domain containing 1
Calculated Molecular Weight 827 aa, 88 kDa
Observed Molecular Weight 88-103 kDa
GenBank Accession NumberBC036557
Gene Symbol CARNS1
Gene ID (NCBI) 57571
RRIDAB_2880211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CARNS1 antibody 25725-1-APDownload protocol
IHC protocol for CARNS1 antibody 25725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Transl Stroke Res

Nicotine Exposure Along with Oral Contraceptive Treatment in Female Rats Exacerbates Post-cerebral Ischemic Hypoperfusion Potentially via Altered Histamine Metabolism.

Authors - Nathan d'Adesky
View Article
ratWB

Neurochem Res

Carnosine Reduces Oxidative Stress and Reverses Attenuation of Righting and Postural Reflexes in Rats with Thioacetamide-Induced Liver Failure.

Authors - Krzysztof Milewski
View Article