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ATP6V1G3 Polyclonal antibody

ATP6V1G3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19523-1-AP

Synonyms

ATP6G3, ATP6V1G3, V ATPase 13 kDa subunit 3, V ATPase subunit G 3, Vma10

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ATP6V1G3 Polyclonal antibody (19523-1-AP)

    WB analysis of HEK-293 using 19523-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ATP6V1G3 Polyclonal antibody (19523-1-AP)

    WB analysis of mouse kidney using 19523-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using ATP6V1G3 Polyclonal antibody (19523-1-AP)

    IP experiment of mouse kidney using 19523-1-AP

    IP result of anti-ATP6V1G3 (IP:19523-1-AP, 4ug; Detection:19523-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP6V1G3 Polyclonal antibody (19523-1-AP)

    IHC staining of human kidney using 19523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP6V1G3 Polyclonal antibody (19523-1-AP)

    IHC staining of human kidney using 19523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP6V1G3 Polyclonal antibody (19523-1-AP)

    IHC staining of human kidney using 19523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP6V1G3 Polyclonal antibody (19523-1-AP)

    IHC staining of human kidney using 19523-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19523-1-AP (ATP6V1G3 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19523-1-AP targets ATP6V1G3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberNM_133326
Gene Symbol ATP6V1G3
Gene ID (NCBI) 127124
RRIDAB_10642838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1G3,also named as ATP6G3, belongs to the V-ATPase G subunit family. ATP6V1G3 is a catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Protocols

Product Specific Protocols
WB protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
IHC protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
IP protocol for ATP6V1G3 antibody 19523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols