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  • Featured Product
  • KD/KO Validated

ATG5 Polyclonal antibody

ATG5 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 10181-2-AP

Synonyms

Apoptosis-specific protein, Apoptosis specific protein, APG5-like, APG5L, APG5 LIKE

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG5 Polyclonal antibody (10181-2-AP)

    WB analysis using 10181-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ATG5 Polyclonal antibody (10181-2-AP)

    WB analysis of HeLa using 10181-2-AP

    WB result of ATG5 antibody (10181-2-AP; 1:2000; room temperature for 1.5 hours) with wild-type and ATG5 knockout HeLa cells.

  • Western Blot (WB) analysis of various lysates using ATG5 Polyclonal antibody (10181-2-AP)

    WB analysis using 10181-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ATG5 Polyclonal antibody (10181-2-AP)

    WB analysis of mouse kidney using 10181-2-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATG5 Polyclonal antibody (10181-2-AP)

    IP experiment of HeLa using 10181-2-AP

    IP result of anti-ATG5 (IP:10181-2-AP, 4ug; Detection:10181-2-AP 1:3000) with HeLa cells lysate 1760 ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ATG5 Polyclonal antibody (10181-2-AP)

    IHC staining of human gliomas using 10181-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ATG5 Polyclonal antibody (10181-2-AP)

    IHC staining of human gliomas using 10181-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ATG5 Polyclonal antibody (10181-2-AP)

    IHC staining of human gliomas using 10181-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ATG5 Polyclonal antibody (10181-2-AP)

    IHC staining of human gliomas using 10181-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, mouse kidney tissue, HepG2 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10181-2-AP targets ATG5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, monkey, chicken, bovine, hamster, goat, grouper
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG5 fusion protein Ag0214
Full Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa, 40-45 kDa, 50-55 kDa
GenBank Accession NumberBC002699
Gene Symbol ATG5
Gene ID (NCBI) 9474
RRIDAB_2062045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

Protocols

Product Specific Protocols
WB protocol for ATG5 antibody 10181-2-APDownload protocol
IHC protocol for ATG5 antibody 10181-2-APDownload protocol
IP protocol for ATG5 antibody 10181-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
View Article
humanWB

Cell Res

Degradation of lipid droplets by chimeric autophagy-tethering compounds.

Authors - Yuhua Fu
  • KO Validated
View Article
humanWB

Mol Cell

Syntaxin 13, a Genetic Modifier of Mutant CHMP2B in Frontotemporal Dementia, Is Required for Autophagosome Maturation.

Authors - Lu Yubing Y
  • KD Validated
View Article
humanWB

Adv Sci (Weinh)

SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle

Authors - Xiaowei She
View Article
human

Autophagy

Autophagy mediates the clearance of oligodendroglial SNCA/alpha-synuclein and TPPP/p25A in multiple system atrophy models

Authors - Panagiota Mavroeidi
View Article
mouseWB

J Clin Invest

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Authors - Shanshan Zhong
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Priya (Verified Customer) (08-09-2023)

Used for Caco2 cells and mice colon tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon
FH

Kamal (Verified Customer) (06-29-2023)

Works well with TBST. Atg5-12 complex (56 kDa) and Atg5 (33kDa) bands came out nice and clear.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse Liver tissue
FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon
FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells and mice tissue

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 1000
  • Cell Tissue Type: Caco2, Mice colon
FH

Benjamin (Verified Customer) (01-07-2020)

Works well in western blot in 3% milk TBS-T

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK239 Mammalian Cell Lysate