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ATAD5 Polyclonal antibody

ATAD5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24954-1-AP

Synonyms

ATAD5, C17orf41, FRAG1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ATAD5 Polyclonal antibody (24954-1-AP)

    WB analysis of Jurkat using 24954-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24954-1-AP (ATAD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ATAD5 Polyclonal antibody (24954-1-AP)

    WB analysis of HeLa using 24954-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24954-1-AP (ATAD5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24954-1-AP is ATAD5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATAD5 fusion protein Ag20498
Full Name ATPase family, AAA domain containing 5
Calculated Molecular Weight 1844 aa, 208 kDa
Observed Molecular Weight 135-150 kDa
GenBank Accession NumberBC015051
Gene Symbol ATAD5
Gene ID (NCBI) 79915
RRIDAB_2879819
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATAD5, also named as C17orf41 and FRAG1, is involved in DNA damage response. It plays a role in PCNA deubiquitination. ATAD5 regulates the cycle of DNA replication factories. ATAD5 has two isoforms with MW 208 kDa and 135 kDa.

Protocols

Product Specific Protocols
WB protocol for ATAD5 antibody 24954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols