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ASNA1 Monoclonal antibody

ASNA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, chicken

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B7C11

Cat no : 66346-1-Ig

Synonyms

TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASNA1 Monoclonal antibody (66346-1-Ig)

    WB analysis using 66346-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ASNA1 Monoclonal antibody (66346-1-Ig)

    WB analysis using 66346-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ASNA1 Monoclonal antibody (66346-1-Ig)

    WB analysis using 66346-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ASNA1 Monoclonal antibody (66346-1-Ig)

    WB analysis using 66346-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using ASNA1 Monoclonal antibody (66346-1-Ig)

    WB analysis of Neuro-2a using 66346-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using ASNA1 Monoclonal antibody (66346-1-Ig)

    WB analysis of fetal human brain using 66346-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using ASNA1 Monoclonal antibody (66346-1-Ig)

    WB analysis of pig brain using 66346-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ASNA1 Monoclonal antibody (66346-1-Ig)

    WB analysis of HEK-293 using 66346-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ASNA1 Monoclonal antibody (66346-1-Ig)

    IHC staining of human lung cancer using 66346-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66346-1-Ig (ASNA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ASNA1 Monoclonal antibody (66346-1-Ig)

    IHC staining of human lung cancer using 66346-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66346-1-Ig (ASNA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ASNA1 Monoclonal antibody (66346-1-Ig)

    IF Staining of HEK-293 using 66346-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ASNA1 antibody (66346-1-Ig, Clone: 1B7C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ASNA1 Monoclonal antibody (66346-1-Ig)

    IF Staining of HEK-293 using 66346-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ASNA1 antibody (66346-1-Ig, Clone: 1B7C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inNeuro-2a cells, fetal human brain tissue, pig brain tissue, HEK-293 cells, HeLa cells, rat brain tissue, rabbit brain tissue, mouse brain tissue, chicken brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66346-1-Ig targets ASNA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, chicken samples.

Tested Reactivity human, mouse, rat, pig, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASNA1 fusion protein Ag7936
Full Name arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Calculated Molecular Weight 38.7 kDa
Observed Molecular Weight 37-41 kDa
GenBank Accession NumberBC002651
Gene Symbol ASNA1
Gene ID (NCBI) 439
RRIDAB_2881726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

Protocols

Product Specific Protocols
WB protocol for ASNA1 antibody 66346-1-IgDownload protocol
IHC protocol for ASNA1 antibody 66346-1-IgDownload protocol
IF protocol for ASNA1 antibody 66346-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols