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ASMTL Polyclonal antibody

ASMTL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14981-1-AP

Synonyms

N-acetylserotonin O-methyltransferase-like protein, dTTPase/UTPase, dTTP/UTP pyrophosphatase, ASTML, ASMTLY

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using ASMTL Polyclonal antibody (14981-1-AP)

    WB analysis of human testis using 14981-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ASMTL Polyclonal antibody (14981-1-AP)

    WB analysis of A549 using 14981-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ASMTL Polyclonal antibody (14981-1-AP)

    WB analysis of HeLa using 14981-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ASMTL Polyclonal antibody (14981-1-AP)

    WB analysis of L02 using 14981-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ASMTL Polyclonal antibody (14981-1-AP)

    WB analysis of HL-60 using 14981-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using ASMTL Polyclonal antibody (14981-1-AP)

    WB analysis of human testis using 14981-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14981-1-AP (ASMTL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ASMTL Polyclonal antibody (14981-1-AP)

    IHC staining of human prostate cancer using 14981-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14981-1-AP (ASMTL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ASMTL Polyclonal antibody (14981-1-AP)

    IHC staining of human prostate cancer using 14981-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14981-1-AP (ASMTL antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ASMTL Polyclonal antibody (14981-1-AP)

    IF Staining of A549 using 14981-1-AP

    Immunofluorescent analysis of A549 cells using 14981-1-AP (ASMTL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman testis tissue, A549 cells, HeLa cells, HL-60 cells, L02 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14981-1-AP targets ASMTL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASMTL fusion protein Ag6894
Full Name acetylserotonin O-methyltransferase-like
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69-75 kDa
GenBank Accession NumberBC002508
Gene Symbol ASMTL
Gene ID (NCBI) 8623
RRIDAB_2059989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ASMTL antibody 14981-1-APDownload protocol
IHC protocol for ASMTL antibody 14981-1-APDownload protocol
IF protocol for ASMTL antibody 14981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols