Various lysates were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 22025-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 22025-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 22025-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 22025-1-AP
IP result of anti-ARPC5L (IP:22025-1-AP, 4ug; Detection:22025-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse brain tissue, human brain tissue, NIH/3T3 cells, mouse lung tissue, rat brain tissue
Positive IP detected in
mouse brain tissue
Positive IHC detected in
human placenta tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
MCF-7 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:4000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ARPC5L, also known as ARC16-2, belongs to the ARPC5 family. ARPC5L is involved in Arp2/3 complex-mediated actin nucleation and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. ARPC5L-containing Arp2/3 complexes are required for the peripheral positioning of nuclei in myofibers (PMID: 28892082). The molecular mass of ARPC5L is 17-20 kDa.
Various lysates were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 22025-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 22025-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 22025-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human placenta using 22025-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 22025-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cerebellum using 22025-1-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cerebellum using 22025-1-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse brain using 22025-1-AP
IP result of anti-ARPC5L (IP:22025-1-AP, 4ug; Detection:22025-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IF/ICC Figures
IF Staining of MCF-7 using 22025-1-AP
Immunofluorescent analysis of MCF-7 cells using 22025-1-AP (ARPC5L antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ARPC5L Polyclonal antibody
Catalog Number
22025-1-AP
Citations
1
Dilutions
WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
