ARPC4 Polyclonal antibody

Name: ARPC4 Polyclonal antibody
Brand: Proteintech
SKU: 10930-1-AP

ARPC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10930-1-AP

Synonyms

ARC20, Arp2/3 complex 20 kDa subunit, ARPC4, p20 Arc

Validation Data Gallery

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AllWBIHC

WB analysis of mouse brain using 10930-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10930-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of 4T1 using 10930-1-AP
4T1 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of human brain using 10930-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10930-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10930-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 10930-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 10930-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat lymph using 10930-1-AP
rat lymph tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 10930-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 10930-1-AP

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Tested Applications

Positive WB detected in	mouse brain tissue, mouse small intestine tissue, rat lymph tissue, 4T1 cells, human placenta tissue, human brain tissue, mouse cerebellum tissue, MCF-7 cells
Positive IHC detected in	human cervical cancer tissue, human testis tissue, human lung cancer tissue, human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 1 publications below

Product Information

10930-1-AP targets ARPC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARPC4 fusion protein Ag1366
Full Name	actin related protein 2/3 complex, subunit 4, 20kDa
Calculated Molecular Weight	20 kDa
Observed Molecular Weight	19-25 kDa
GenBank Accession Number	BC012596
Gene Symbol	ARPC4
Gene ID (NCBI)	10093
RRID	AB_2059951
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ARP2/3 complex consists of seven subunits: two actin-related proteins (ARP2 and ARP3) and five ARP2/3 complex polypeptides (ARPC1B and ARPC2-5). ARPC4 is a core subunit of the actin-related protein (ARP2/3) complex, which catalyzes the formation of F-actin networks. ARPC4 is essential for the structural integrity of the ARP2/3 complex function. Mutations in ARPC4 cause Developmental delay, language impairment, and ocular abnormalities (DEVLO)(PMID: 35047857).

Protocols

Product Specific Protocols
WB protocol for ARPC4 antibody 10930-1-AP	Download protocol
IHC protocol for ARPC4 antibody 10930-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Life Sci Alliance A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells Authors - Sumana Sharma View Article
human	WB	Biochim Biophys Acta Proteins Proteom Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis. Authors - Guoxuan Qin View Article
human	WB	Drug Resist Updat Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane Authors - Xin Zhang View Article
mouse,bovine	WB,IF	Biomolecules Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model Authors - Bo Fang View Article

ARPC4 Polyclonal antibody

ARPC4 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of mouse brain using 10930-1-AP

WB analysis of MCF-7 using 10930-1-AP

WB analysis of 4T1 using 10930-1-AP

WB analysis of human brain using 10930-1-AP

WB analysis of human placenta using 10930-1-AP

WB analysis of human brain using 10930-1-AP

WB analysis of mouse small intestine using 10930-1-AP

WB analysis of mouse cerebellum using 10930-1-AP

WB analysis of rat lymph using 10930-1-AP

IHC staining of human lung cancer using 10930-1-AP

IHC staining of human testis using 10930-1-AP

IHC staining of human cervical cancer using 10930-1-AP

IHC staining of human cervical cancer using 10930-1-AP

IHC staining of human urothelial carcinoma using 10930-1-AP

IHC staining of human testis using 10930-1-AP

IHC staining of human testis using 10930-1-AP

IHC staining of human testis using 10930-1-AP

IHC staining of human lung cancer using 10930-1-AP

IHC staining of human lung cancer using 10930-1-AP

IHC staining of human lung cancer using 10930-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells

Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis.

Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane

Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model