ARL13B Monoclonal antibody
ARL13B Monoclonal Antibody for WB, IF/ICC, IF-Fro, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, pig, canine and More (1)
Applications
WB, IHC, IF/ICC, IF-Fro, ELISA
Conjugate
Unconjugated
CloneNo.
1H6C3
Cat no : 66739-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, NCCIT cell |
| Positive IF-Fro detected in | mouse heart tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 18 publications below |
Product Information
66739-1-Ig targets ARL13B in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human, mouse, pig, canine samples.
| Tested Reactivity | human, mouse, pig, canine |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ARL13B fusion protein Ag12031 |
| Full Name | ADP-ribosylation factor-like 13B |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC094725 |
| Gene Symbol | ARL13B |
| Gene ID (NCBI) | 200894 |
| RRID | AB_2882088 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARL13B antibody 66739-1-Ig | Download protocol |
| IF protocol for ARL13B antibody 66739-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex | ||
Sci Rep Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts. | ||
eNeuro CCP1, a Tubulin Deglutamylase, Increases Survival of Rodent Spinal Cord Neurons following Glutamate-Induced Excitotoxicity. | ||
Life Sci Alliance HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Lindsey (Verified Customer) (05-24-2021) | I much prefer the Rabbit polyclonal ARL13B (Cat#17711-I-AP) but it is really nice to have an Arl13B Mouse monoclonal for use in co-stains with other targets. I wish it didn't have to be used at such a high working concentration!
 |
FH Feng-Qian (Verified Customer) (04-04-2019) | This antibody works for detecting primary cilia in MEFs with low background. The intensity is lower than the Arl13B rabbit antibody (17711-1-AP) in general but specific.
|
FH Francesc (Verified Customer) (11-07-2018) | This antibody works beautifully, staining cilia with high intensity and specificity, as expected for ARL13B.The staining with 66739-1-Ig appears as good as that obtained with the highly validated Proteintech's rabbit anti-ARL13B (17711-1-AP).This mouse anti-ARL13B makes for a great ciliary marker.
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