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  • Featured Product
  • KD/KO Validated

ARID3A Polyclonal antibody

ARID3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14068-1-AP

Synonyms

DRIL1, Dead ringer-like protein 1, BRIGHT, B-cell regulator of IgH transcription, AT-rich interactive domain-containing protein 3A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARID3A Polyclonal antibody (14068-1-AP)

    WB analysis using 14068-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14068-1-AP (ARID3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ARID3A Polyclonal antibody (14068-1-AP)

    WB analysis of K-562 using 14068-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14068-1-AP (ARID3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using ARID3A Polyclonal antibody (14068-1-AP)

    IP experiment of K-562 using 14068-1-AP

    IP result of anti-ARID3A (IP:14068-1-AP, 4ug; Detection:14068-1-AP 1:1000) with K-562 cells lysate 3200ug.

  • Immunoprecipitation (IP) experiment of K-562 cells using ARID3A Polyclonal antibody (14068-1-AP)

    IP experiment of K-562 using 14068-1-AP

    IP result of anti-ARID3A (IP:14068-1-AP, 4ug; Detection:14068-1-AP 1:1000) with K-562 cells lysate 3680 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ARID3A Polyclonal antibody (14068-1-AP)

    IHC staining of human colon cancer using 14068-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ARID3A Polyclonal antibody (14068-1-AP)

    IHC staining of human colon cancer using 14068-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ARID3A Polyclonal antibody (14068-1-AP)

    IHC staining of human colon cancer using 14068-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ARID3A Polyclonal antibody (14068-1-AP)

    IHC staining of human colon cancer using 14068-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARID3A Polyclonal antibody (14068-1-AP)

    IF Staining of HepG2 using 14068-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARID3A antibody (14068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARID3A Polyclonal antibody (14068-1-AP)

    IF Staining of HepG2 using 14068-1-AP

    Immunofluorescent analysis of HepG2 cells, using ARID3A antibody 14068-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inK-562 cells, mouse placenta tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14068-1-AP targets ARID3A in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARID3A fusion protein Ag5124
Full Name AT rich interactive domain 3A (BRIGHT-like)
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC060828
Gene Symbol ARID3A
Gene ID (NCBI) 1820
RRIDAB_2060390
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).

Protocols

Product Specific Protocols
WB protocol for ARID3A antibody 14068-1-APDownload protocol
IHC protocol for ARID3A antibody 14068-1-APDownload protocol
IF protocol for ARID3A antibody 14068-1-APDownload protocol
IP protocol for ARID3A antibody 14068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Rep Med

Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application

Authors - Xiaoxue Ren
View Article
mouseIHC

PLoS Genet

Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2.

Authors - Blair B Madison
View Article
humanWB,IP

Carcinogenesis

ARID3A promotes the development of colorectal cancer by upregulating AURKA.

Authors - Jing Tang
  • KD Validated
View Article
humanWB,IHC

Ann Surg Oncol

High Expression of AT-Rich Interactive Domain 3A (ARID3A) is Associated with Good Prognosis in Colorectal Carcinoma.

Authors - Meiying Song
View Article
human,mouseWB,IF

Cell Biol Int

ARID3A plays a key regulatory role in palmitic acid-stimulated milk fat synthesis in mouse mammary epithelial cells

Authors - Xudong Guo
  • KD Validated
View Article
humanWB,ChIP

Cell Biol Int

ARID3A promotes the chemosensitivity of colon cancer by inhibiting AKR1C3.

Authors - Yafei Li
View Article