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- KD/KO Validated
ARHGEF4 Polyclonal antibody
ARHGEF4 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 55213-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, multi-cells/tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
55213-1-AP targets ARHGEF4 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | Rho guanine nucleotide exchange factor (GEF) 4 |
| Calculated Molecular Weight | 79 kDa |
| Observed Molecular Weight | 72 kDa |
| GenBank Accession Number | NM_015320 |
| Gene Symbol | ARHGEF4 |
| Gene ID (NCBI) | 50649 |
| RRID | AB_2721023 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGEF4 antibody 55213-1-AP | Download protocol |
| IHC protocol for ARHGEF4 antibody 55213-1-AP | Download protocol |
| IP protocol for ARHGEF4 antibody 55213-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Peptidomimetic inhibitors of APC-Asef interaction block colorectal cancer migration. | ||
J Neurosci Temporal Profiling of Astrocyte Precursors Reveals Parallel Roles for Asef during Development and after Injury.
| ||
iScience Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma | ||
Int J Oncol ARHGEF4 predicts poor prognosis and promotes cell invasion by influencing ERK1/2 and GSK-3α/β signaling in pancreatic cancer. | ||
Biol Reprod Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis. | ||
Cell Adh Migr ARHGEF4-mediates the actin cytoskeleton reorganization of hepatic stellate cells in 3-dimensional collagen matrices.
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