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- KD/KO Validated
ARHGEF10 Polyclonal antibody
ARHGEF10 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11112-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
11112-1-AP targets ARHGEF10 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ARHGEF10 fusion protein Ag1605 |
| Full Name | Rho guanine nucleotide exchange factor (GEF) 10 |
| Calculated Molecular Weight | 147 kDa |
| GenBank Accession Number | BC036809 |
| Gene Symbol | ARHGEF10 |
| Gene ID (NCBI) | 9639 |
| RRID | AB_2059754 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARHGEF10(Rho guanine nucleotide exchange factor 10), also named as KIAA0294, is a member of the family of Rho guanine nucleotide exchange factors (GEFs), which are implicated in neural morphogenesis and connectivity and regulate the activity of small Rho GTPases by catalyzing the exchange of bound GDP by GTP(PMID:14508709). The deduced 1,121-amino acid protein shares significant similarity with cell signaling proteins(PMID:9205841). Defects in ARHGEF10 are the cause of slowed nerve conduction velocity (SNCV)(PMID:14508709). It has 5 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARHGEF10 antibody 11112-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Thromb Haemost ARHGEF10 knockout inhibits platelet aggregation and protects mice from thrombus formation.
| ||
