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ARHGAP27 Polyclonal antibody

ARHGAP27 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22077-1-AP

Synonyms

SH3 domain-containing protein 20, Rho-type GTPase-activating protein 27, Rho GTPase-activating protein 27, PP905, CIN85-associated multi-domain-containing Rho GTPase-activating protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using ARHGAP27 Polyclonal antibody (22077-1-AP)

    WB analysis of A549 using 22077-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22077-1-AP (ARHGAP27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARHGAP27 Polyclonal antibody (22077-1-AP)

    IHC staining of human breast cancer using 22077-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARHGAP27 Polyclonal antibody (22077-1-AP)

    IHC staining of human breast cancer using 22077-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARHGAP27 Polyclonal antibody (22077-1-AP)

    IHC staining of human pancreas using 22077-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARHGAP27 Polyclonal antibody (22077-1-AP)

    IHC staining of human pancreas using 22077-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARHGAP27 Polyclonal antibody (22077-1-AP)

    IF Staining of HeLa using 22077-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

22077-1-AP targets ARHGAP27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP27 fusion protein Ag17225
Full Name Rho GTPase activating protein 27
Calculated Molecular Weight 548 aa, 62 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC101388
Gene Symbol ARHGAP27
Gene ID (NCBI) 201176
RRIDAB_2878985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARHGAP27 antibody 22077-1-APDownload protocol
IHC protocol for ARHGAP27 antibody 22077-1-APDownload protocol
IF protocol for ARHGAP27 antibody 22077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Targeting ALDOA to modulate tumorigenesis and energy metabolism in retinoblastoma

Authors - Yinghao Wang
View Article