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  • Featured Product
  • KD/KO Validated

ARHGAP25 Polyclonal antibody

ARHGAP25 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14349-1-AP

Synonyms

Rho-type GTPase-activating protein 25, Rho GTPase-activating protein 25, KIAA0053

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGAP25 Polyclonal antibody (14349-1-AP)

    WB analysis using 14349-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14349-1-AP (ARHGAP25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ARHGAP25 Polyclonal antibody (14349-1-AP)

    WB analysis of Jurkat using 14349-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14349-1-AP (ARHGAP25 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ARHGAP25 Polyclonal antibody (14349-1-AP)

    WB analysis of Jurkat using 14349-1-AP

    WB result of ARHGAP25 antibody (14349-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARHGAP25 transfected Jurkat cells.

  • Immunoprecipitation (IP) experiment of Jurkat cells using ARHGAP25 Polyclonal antibody (14349-1-AP)

    IP experiment of Jurkat using 14349-1-AP

    IP result of anti-ARHGAP25 (IP:14349-1-AP, 4ug; Detection:14349-1-AP 1:500) with Jurkat cells lysate 1000ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ARHGAP25 Polyclonal antibody (14349-1-AP)

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ARHGAP25 Polyclonal antibody (14349-1-AP)

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ARHGAP25 Polyclonal antibody (14349-1-AP)

    IHC staining of human tonsillitis using 14349-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14349-1-AP (ARHGAP25 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, Ramos cells, THP-1 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14349-1-AP targets ARHGAP25 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP25 fusion protein Ag5691
Full Name Rho GTPase activating protein 25
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC039591
Gene Symbol ARHGAP25
Gene ID (NCBI) 9938
RRIDAB_2059273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP25 (Rho GTPase-activating protein 25) also known as KAIA0053, belongs to a family of Rho GTPase-modulating proteins which are implicated in actin remodeling,cell polarity, and cell migration. ARHGAP25 has a key role in the pathomechanism of autoantibody-induced arthritis in which it regulates inflammation.

Protocols

Product Specific Protocols
WB protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IHC protocol for ARHGAP25 antibody 14349-1-APDownload protocol
IP protocol for ARHGAP25 antibody 14349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Carcinogenesis

ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop

Authors - Sijia Han
  • KD Validated
View Article