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ARHGAP21 Polyclonal antibody
ARHGAP21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 22183-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells |
| Positive IHC detected in | human ovary cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
22183-1-AP targets ARHGAP21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ARHGAP21 fusion protein Ag16515 |
| Full Name | Rho GTPase activating protein 21 |
| Calculated Molecular Weight | 1957 aa, 217 kDa |
| Observed Molecular Weight | 250 kDa |
| GenBank Accession Number | BC118915 |
| Gene Symbol | ARHGAP21 |
| Gene ID (NCBI) | 57584 |
| RRID | AB_2879018 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ARHGAP21 antibody 22183-1-AP | Download protocol |
| IHC protocol for ARHGAP21 antibody 22183-1-AP | Download protocol |
| IF protocol for ARHGAP21 antibody 22183-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Phosphorylation-dependent recognition of diverse protein targets by the cryptic GK domain of MAGI MAGUKs
| ||
Elife PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Joleen (Verified Customer) (06-03-2019) | first 2 lanes are whole cell lysate. ARHGAP21doesn't show up prominently but does show up in last 2 lanes which are purified biotinylated proteins surrounding out protein of interest. This seems to indicate that the antibody works but just that the cells we use express levels that are below the level of detection unless it is enriched (such as with our purification protocol)
 |
