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ARHGAP1 Polyclonal antibody

ARHGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11169-1-AP

Synonyms

p50-RhoGAP, p50rhoGAP, p50 RhoGAP, GTPase-activating protein rhoGAP, CDC42GAP

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using ARHGAP1 Polyclonal antibody (11169-1-AP)

    WB analysis of RAW 264.7 using 11169-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using ARHGAP1 Polyclonal antibody (11169-1-AP)

    WB analysis of PC-12 using 11169-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human duodenum tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    WB analysis of human duodenum using 11169-1-AP

    human duodenum tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human pancreas tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    WB analysis of human pancreas using 11169-1-AP

    human pancreas tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    WB analysis of human heart using 11169-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    IHC staining of human pancreas using 11169-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    IHC staining of human pancreas using 11169-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ARHGAP1 Polyclonal antibody (11169-1-AP)

    IHC staining of human pancreas cancer using 11169-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11169-1-AP (ARHGAP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARHGAP1 Polyclonal antibody (11169-1-AP)

    IF Staining of HeLa using 11169-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11169-1-AP (ARHGAP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inRAW 264.7 cells, human duodenum tissue, human heart tissue, human pancreas tissue, PC-12 cells
Positive IHC detected inhuman pancreas tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11169-1-AP targets ARHGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP1 fusion protein Ag1651
Full Name Rho GTPase activating protein 1
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC018118
Gene Symbol ARHGAP1
Gene ID (NCBI) 392
RRIDAB_2289862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARHGAP1 antibody 11169-1-APDownload protocol
IHC protocol for ARHGAP1 antibody 11169-1-APDownload protocol
IF protocol for ARHGAP1 antibody 11169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols