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APOBEC3G Polyclonal antibody

APOBEC3G Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10608-1-AP

Synonyms

A3G, APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using APOBEC3G Polyclonal antibody (10608-1-AP)

    WB analysis of mouse colon using 10608-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 10608-1-AP (APOBEC3G Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using APOBEC3G Polyclonal antibody (10608-1-AP)

    IP experiment of COLO 320 using 10608-1-AP

    IP result of anti-APOBEC3G (IP:10608-1-AP, 4ug; Detection:10608-1-AP 1:300) with COLO 320 cells lysate 2240ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using APOBEC3G Polyclonal antibody (10608-1-AP)

    IHC staining of human testis using 10608-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using APOBEC3G Polyclonal antibody (10608-1-AP)

    IHC staining of human testis using 10608-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using APOBEC3G Polyclonal antibody (10608-1-AP)

    IHC staining of human colon cancer using 10608-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using APOBEC3G Polyclonal antibody (10608-1-AP)

    IHC staining of human colon cancer using 10608-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using APOBEC3G Polyclonal antibody (10608-1-AP)

    IF Staining of MCF-7 using 10608-1-AP

    Immunofluorescent analysis of MCF-7 cells using 10608-1-AP (APOBEC3G antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse colon tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman testis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10608-1-AP targets APOBEC3G in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOBEC3G fusion protein Ag0887
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC024268
Gene Symbol APOBEC3G
Gene ID (NCBI) 60489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

Publications

SpeciesApplicationTitle
humanWB

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
View Article
humanWB,IHC

J Clin Invest

APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis.

Authors - Ding Qingqing Q
View Article
humanWB

Cancers (Basel)

Overexpression of NNMT in Glioma Aggravates Tumor Cell Progression: An Emerging Therapeutic Target

Authors - Wei Sun
View Article