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AARE Polyclonal antibody

AARE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14758-1-AP

Synonyms

APEH, Acyl-peptide hydrolase, Acylaminoacyl-peptidase, Acylaminoacyl peptidase, Acylamino-acid-releasing enzyme

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using AARE Polyclonal antibody (14758-1-AP)

    WB analysis using 14758-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14758-1-AP (AARE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using AARE Polyclonal antibody (14758-1-AP)

    IHC staining of human liver using 14758-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 14758-1-AP (AARE antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using AARE Polyclonal antibody (14758-1-AP)

    IHC staining of human liver using 14758-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 14758-1-AP (AARE antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AARE Polyclonal antibody (14758-1-AP)

    IF Staining of HepG2 using 14758-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AARE antibody (14758-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, human plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14758-1-AP targets AARE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AARE fusion protein Ag6511
Full Name N-acylaminoacyl-peptide hydrolase
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 66-80 kDa
GenBank Accession NumberBC000362
Gene Symbol APEH
Gene ID (NCBI) 327
RRIDAB_2057811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APH, (APEH, acylpeptide hydrolase), which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. A range of molecular masses have been reported for APH by previous investigators, including 80 kD, 75 kD and 69 kD. This antibody can recognize at least 2 isoforms of APH.

Protocols

Product Specific Protocols
WB protocol for AARE antibody 14758-1-APDownload protocol
IHC protocol for AARE antibody 14758-1-APDownload protocol
IF protocol for AARE antibody 14758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

mSphere

Internalization of Erythrocyte Acylpeptide Hydrolase Is Required for Asexual Replication of Plasmodium falciparum.

Authors - Rubayet Elahi
View Article