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APBB2 Polyclonal antibody

APBB2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13177-1-AP

Synonyms

FE65L1, FE65L, Protein Fe65-like 1, Protein Fe65 like 1, Amyloid-beta

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using APBB2 Polyclonal antibody (13177-1-AP)

    WB analysis of human brain using 13177-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13177-1-AP (APBB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using APBB2 Polyclonal antibody (13177-1-AP)

    IF Staining of HeLa using 13177-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13177-1-AP (APBB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13177-1-AP targets APBB2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APBB2 fusion protein Ag3844
Full Name amyloid beta (A4) precursor protein-binding, family B, member 2
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 83-95 kDa
GenBank Accession NumberBC027946
Gene Symbol APBB2
Gene ID (NCBI) 323
RRIDAB_10638434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APBB2 antibody 13177-1-APDownload protocol
IF protocol for APBB2 antibody 13177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols