• Featured Product
  • KD/KO Validated

Aldehyde oxidase Polyclonal antibody

Aldehyde oxidase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 19495-1-AP

Synonyms

EC:1.2.3.1, EC:1.17.3.-, Azaheterocycle hydroxylase, AOX1, AOX 1



Tested Applications

Positive WB detected inmouse liver tissue, mouse lung tissue, mouse pancreas tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19495-1-AP targets Aldehyde oxidase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name aldehyde oxidase 1
Calculated Molecular Weight 148 kDa
Observed Molecular Weight 148 kDa
GenBank Accession NumberNM_001159
Gene Symbol AOX1
Gene ID (NCBI) 316
RRIDAB_10640579
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Aldehyde oxidase antibody 19495-1-APDownload protocol
IHC protocol for Aldehyde oxidase antibody 19495-1-APDownload protocol
IF protocol for Aldehyde oxidase antibody 19495-1-APDownload protocol
IP protocol for Aldehyde oxidase antibody 19495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO Rep

Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice.

Authors - Laura M Zidek
humanWB,IHC

Oncogene

Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression.

Authors - Venkatrao Vantaku
  • KD Validated
humanIF

Am J Obstet Gynecol

Evidence that fetal death is associated with placental aging.

Authors - Kaushik Maiti
mouseWB

Free Radic Biol Med

PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.

Authors - Xue Chen
humanWB

Front Immunol

Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma.

Authors - Shun-Li Peng
humanWB

Cell Signal

Aldehyde oxidase 1 promotes gallbladder carcinogenesis through ROS-mediated activation of the Wnt/β-catenin pathway

Authors - Ziheng Yuan