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ANKS3 Polyclonal antibody

ANKS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 24058-1-AP

Synonyms

ANKS3, KIAA1977

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ANKS3 Polyclonal antibody (24058-1-AP)

    WB analysis of mouse liver using 24058-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24058-1-AP (ANKS3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ANKS3 Polyclonal antibody (24058-1-AP)

    WB analysis of human brain using 24058-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 24058-1-AP (ANKS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ANKS3 Polyclonal antibody (24058-1-AP)

    IHC staining of human breast cancer using 24058-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ANKS3 Polyclonal antibody (24058-1-AP)

    IHC staining of human hepatocirrhosis using 24058-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ANKS3 Polyclonal antibody (24058-1-AP)

    IHC staining of human breast cancer using 24058-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ANKS3 Polyclonal antibody (24058-1-AP)

    IHC staining of human hepatocirrhosis using 24058-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inmouse liver tissue, human brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24058-1-AP is ANKS3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKS3 fusion protein Ag21252
Full Name ankyrin repeat and sterile alpha motif domain containing 3
Calculated Molecular Weight 656 aa, 72 kDa
Observed Molecular Weight 72 kDa, 63 kDa
GenBank Accession NumberBC050387
Gene Symbol ANKS3
Gene ID (NCBI) 124401
RRIDAB_2879418
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKS3 (ankyrin repeat and sterile alpha motif domain containing 3) is a 656 amino acid protein that contain six ANK repeats and one SAM (sterile alpha motif) domain. Each ANK repeat consists of a 30-34 amino acid sequence that exhibits a helix-turn-helix conformation and functions to mediate protein-protein interactions. The SAM domain is a 70 amino acid domain that also functions to mediate protein-protein interactions for homo- and hetero- oligomerization. Two isoforms of ANKS3 exist due to alternative splicing events.This antibody may recognize both of them.

Protocols

Product Specific Protocols
WB protocol for ANKS3 antibody 24058-1-APDownload protocol
IHC protocol for ANKS3 antibody 24058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IP,CoIP

PLoS Biol

Bicc1 ribonucleoprotein complexes specifying organ laterality are licensed by ANKS6-induced structural remodeling of associated ANKS3

Authors - Benjamin Rothé
View Article