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ANKRD57 Polyclonal antibody

ANKRD57 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24033-1-AP

Synonyms

ANKRD57, ankyrin repeat domain 57, C2orf26

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD57 Polyclonal antibody (24033-1-AP)

    WB analysis using 24033-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24033-1-AP (ANKRD57 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ANKRD57 Polyclonal antibody (24033-1-AP)

    IP experiment of HeLa using 24033-1-AP

    IP result of anti-ANKRD57 (IP:24033-1-AP, 3ug; Detection:24033-1-AP 1:1000) with HeLa cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ANKRD57 Polyclonal antibody (24033-1-AP)

    IHC staining of human cerebellum using 24033-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ANKRD57 Polyclonal antibody (24033-1-AP)

    IHC staining of human cerebellum using 24033-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ANKRD57 Polyclonal antibody (24033-1-AP)

    IHC staining of human cervical cancer using 24033-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using ANKRD57 Polyclonal antibody (24033-1-AP)

    IHC staining of human cervical cancer using 24033-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cerebellum tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 24033-1-AP is ANKRD57 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD57 fusion protein Ag21238
Full Name ankyrin repeat domain 57
Calculated Molecular Weight 525 aa, 56 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC036652
Gene Symbol ANKRD57
Gene ID (NCBI) 65124
RRIDAB_2879412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ANKRD57 antibody 24033-1-APDownload protocol
IHC protocol for ANKRD57 antibody 24033-1-APDownload protocol
IP protocol for ANKRD57 antibody 24033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

A DNA-Methylation-Driven Genes Based Prognostic Signature Reveals Immune Microenvironment in Pancreatic Cancer.

Authors - Mingjia Xiao
View Article