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ANKRD54 Polyclonal antibody

ANKRD54 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25738-1-AP

Synonyms

ANKRD54, ankyrin repeat domain 54

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ANKRD54 Polyclonal antibody (25738-1-AP)

    WB analysis of mouse testis using 25738-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25738-1-AP (ANKRD54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ANKRD54 Polyclonal antibody (25738-1-AP)

    WB analysis of K-562 using 25738-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25738-1-AP (ANKRD54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using ANKRD54 Polyclonal antibody (25738-1-AP)

    WB analysis of PC-3 using 25738-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25738-1-AP (ANKRD54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ANKRD54 Polyclonal antibody (25738-1-AP)

    IHC staining of mouse testis using 25738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25738-1-AP (ANKRD54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ANKRD54 Polyclonal antibody (25738-1-AP)

    IHC staining of mouse testis using 25738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25738-1-AP (ANKRD54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ANKRD54 Polyclonal antibody (25738-1-AP)

    IHC staining of human ovary using 25738-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25738-1-AP (ANKRD54 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using ANKRD54 Polyclonal antibody (25738-1-AP)

    IHC staining of human ovary using 25738-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25738-1-AP (ANKRD54 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue, K-562 cells, PC-3 cells
Positive IHC detected inmouse testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25738-1-AP targets ANKRD54 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD54 fusion protein Ag22759
Full Name ankyrin repeat domain 54
Calculated Molecular Weight 300 aa, 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC066909
Gene Symbol ANKRD54
Gene ID (NCBI) 129138
RRIDAB_2880218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ANKRD54 antibody 25738-1-APDownload protocol
IHC protocol for ANKRD54 antibody 25738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols