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ANKRD50 Polyclonal antibody

ANKRD50 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24032-1-AP

Synonyms

ANKRD50, ankyrin repeat domain 50, KIAA1223

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ANKRD50 Polyclonal antibody (24032-1-AP)

    WB analysis of HEK-293 using 24032-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24032-1-AP (ANKRD50 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using ANKRD50 Polyclonal antibody (24032-1-AP)

    IP experiment of HEK-293 using 24032-1-AP

    IP result of anti-ANKRD50 (IP:24032-1-AP, 5ug; Detection:24032-1-AP 1:300) with HEK-293 cells lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ANKRD50 Polyclonal antibody (24032-1-AP)

    IF Staining of HEK-293 using 24032-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24032-1-AP (ANKRD50 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24032-1-AP is ANKRD50 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD50 fusion protein Ag21237
Full Name ankyrin repeat domain 50
Calculated Molecular Weight 1429 aa, 156 kDa
Observed Molecular Weight 137-156 kDa
GenBank Accession NumberBC024725
Gene Symbol ANKRD50
Gene ID (NCBI) 57182
RRIDAB_2879411
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ANKRD50 antibody 24032-1-APDownload protocol
IF protocol for ANKRD50 antibody 24032-1-APDownload protocol
IP protocol for ANKRD50 antibody 24032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols