ANKRD49 Polyclonal antibody
ANKRD49 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25034-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
25034-1-AP targets ANKRD49 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ANKRD49 fusion protein Ag18295 |
| Full Name | ankyrin repeat domain 49 |
| Calculated Molecular Weight | 239 aa, 27 kDa |
| Observed Molecular Weight | 27-30 kDa |
| GenBank Accession Number | BC017798 |
| Gene Symbol | ANKRD49 |
| Gene ID (NCBI) | 54851 |
| RRID | AB_2879860 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ankyrin repeat domain-containing protein 49 (ANKRD49), an evolutionarily conserved protein highly expressed in the testes, is mainly found in spermatogonia, spermatocytes, and round spermatids. ANKRD49 also participates in the progression of malignant gliomas and gastric cancer in humans. (PMID: 37964204, PMID: 30798416)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANKRD49 antibody 25034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med ANKRD49 promotes the invasion and metastasis of lung adenocarcinoma via a P38/ATF-2 signalling pathway. | ||
Appl Immunohistochem Mol Morphol A Microarray Study on the Expression of ANKRD49 in Lung Squamous Cell Carcinoma and Its Clinicopathologic Significance. | ||
BMC Cancer ANKRD49 promotes the metastasis of NSCLC via activating JNK-ATF2/c-Jun-MMP-2/9 axis |
