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ANKRD39 Polyclonal antibody

ANKRD39 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24027-1-AP

Synonyms

ANKRD39, ankyrin repeat domain 39

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ANKRD39 Polyclonal antibody (24027-1-AP)

    WB analysis of PC-3 using 24027-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24027-1-AP (ANKRD39 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using ANKRD39 Polyclonal antibody (24027-1-AP)

    WB analysis of Transfected HEK-293 using 24027-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 24027-1-AP (ANKRD39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ANKRD39 Polyclonal antibody (24027-1-AP)

    IF Staining of PC-3 using 24027-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 24027-1-AP (ANKRD39 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, Transfected HEK-293 cells
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24027-1-AP is ANKRD39 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD39 fusion protein Ag21210
Full Name ankyrin repeat domain 39
Calculated Molecular Weight 183 aa, 20 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC031303
Gene Symbol ANKRD39
Gene ID (NCBI) 51239
RRIDAB_2879407
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ANKRD39 antibody 24027-1-APDownload protocol
IF protocol for ANKRD39 antibody 24027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols