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ANKRD29 Polyclonal antibody

ANKRD29 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 23999-1-AP

Synonyms

ANKRD29, ankyrin repeat domain 29

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using ANKRD29 Polyclonal antibody (23999-1-AP)

    WB analysis of mouse brain using 23999-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23999-1-AP (ANKRD29 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ANKRD29 Polyclonal antibody (23999-1-AP)

    IP experiment of mouse brain using 23999-1-AP

    IP result of anti-ANKRD29 (IP:23999-1-AP, 3ug; Detection:23999-1-AP 1:300) with mouse brain tissue lysate 2640ug.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23999-1-AP is ANKRD29 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD29 fusion protein Ag21201
Full Name ankyrin repeat domain 29
Calculated Molecular Weight 301 aa, 33 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC030622
Gene Symbol ANKRD29
Gene ID (NCBI) 147463
RRIDAB_2879398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ANKRD29 antibody 23999-1-APDownload protocol
IP protocol for ANKRD29 antibody 23999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

N-terminal cysteine acetylation and oxidation patterns may define protein stability

Authors - Karen C Heathcote
View Article