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ANKRD13D Polyclonal antibody

ANKRD13D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21100-1-AP

Synonyms

ANKRD13D

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ANKRD13D Polyclonal antibody (21100-1-AP)

    WB analysis of Jurkat using 21100-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21100-1-AP (ANKRD13D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using ANKRD13D Polyclonal antibody (21100-1-AP)

    WB analysis of HT-1080 using 21100-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 21100-1-AP (ANKRD13D Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ANKRD13D Polyclonal antibody (21100-1-AP)

    IHC staining of human breast cancer using 21100-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21100-1-AP (ANKRD13D Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ANKRD13D Polyclonal antibody (21100-1-AP)

    IHC staining of human small intestine using 21100-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21100-1-AP (ANKRD13D Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ANKRD13D Polyclonal antibody (21100-1-AP)

    IHC staining of human breast cancer using 21100-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21100-1-AP (ANKRD13D Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ANKRD13D Polyclonal antibody (21100-1-AP)

    IHC staining of human small intestine using 21100-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21100-1-AP (ANKRD13D Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inJurkat cells, HT-1080 cells
Positive IHC detected inhuman small intestine tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21100-1-AP is ANKRD13D Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD13D fusion protein Ag15369
Full Name ankyrin repeat domain 13 family, member D
Calculated Molecular Weight 605 aa, 68 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC110419
Gene Symbol ANKRD13D
Gene ID (NCBI) 338692
RRIDAB_2878813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD13D, also named as Ankyrin repeat domain-containing protein 13D, is a 518 amino acid protein, which contains 4 UIM (ubiquitin-interacting motif) repeats. ANKRD13D localizes in the cell membrane and late endosome. ANKRD13D as a ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. The calculated molecular weight of ANKRD13D is 58kDa, but we decteted a 50kDa protein via western blot. experiment.

Protocols

Product Specific Protocols
WB protocol for ANKRD13D antibody 21100-1-APDownload protocol
IHC protocol for ANKRD13D antibody 21100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols