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ANKRD13C Polyclonal antibody

ANKRD13C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24000-1-AP

Synonyms

ANKRD13C, ankyrin repeat domain 13C, RP4 677H15.5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD13C Polyclonal antibody (24000-1-AP)

    WB analysis using 24000-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24000-1-AP (ANKRD13C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ANKRD13C Polyclonal antibody (24000-1-AP)

    WB analysis of mouse testis using 24000-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24000-1-AP (ANKRD13C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24000-1-AP is ANKRD13C Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD13C fusion protein Ag21202
Full Name ankyrin repeat domain 13C
Calculated Molecular Weight 541 aa, 61 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC028840
Gene Symbol ANKRD13C
Gene ID (NCBI) 81573
RRIDAB_2879399
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD13C, also termed as ankyrin repeat domain 13C, belongs to the ankyrin repeat domain 13 family. ANKRD13C is localized on ER membranes where it binds to DP to control its biogenesis and trafficking. ANKRD13C modulates the expression and maturation of other G pretein coupled receptors as well, indicating that ANKRD13C is a novel regulator of the biogenesis and trafficking of G pretein coupled receptors through the biosynthetic pathway.

Protocols

Product Specific Protocols
WB protocol for ANKRD13C antibody 24000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols