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ANKRD13B Polyclonal antibody

ANKRD13B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25737-1-AP

Synonyms

ANKRD13B, ankyrin repeat domain 13B

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse small intestine tissue using ANKRD13B Polyclonal antibody (25737-1-AP)

    WB analysis of mouse small intestine using 25737-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25737-1-AP (ANKRD13B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ANKRD13B Polyclonal antibody (25737-1-AP)

    IHC staining of human testis using 25737-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25737-1-AP (ANKRD13B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ANKRD13B Polyclonal antibody (25737-1-AP)

    IHC staining of human testis using 25737-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25737-1-AP (ANKRD13B Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse small intestine tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25737-1-AP is ANKRD13B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD13B fusion protein Ag22732
Full Name ankyrin repeat domain 13B
Calculated Molecular Weight 626 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC032554
Gene Symbol ANKRD13B
Gene ID (NCBI) 124930
RRIDAB_2880217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD13B (ankyrin repeat domain 13B) is an ubiquitin-binding protein belongs to the Ankrd 13 family. It specifically recognizes and binds 'Lys-63'-linked ubiquitin, and positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane (PMID: 22298428). This antibody specifically recognises the 70 kDa protein.

Protocols

Product Specific Protocols
WB protocol for ANKRD13B antibody 25737-1-APDownload protocol
IHC protocol for ANKRD13B antibody 25737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols