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ANKRD13A Polyclonal antibody

ANKRD13A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 23998-1-AP

Synonyms

Protein KE03, Ankyrin repeat domain-containing protein 13A, ANKRD13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ANKRD13A Polyclonal antibody (23998-1-AP)

    WB analysis using 23998-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23998-1-AP (ANKRD13A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ANKRD13A Polyclonal antibody (23998-1-AP)

    WB analysis using 23998-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23998-1-AP (ANKRD13A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ANKRD13A Polyclonal antibody (23998-1-AP)

    WB analysis of HepG2 using 23998-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23998-1-AP (ANKRD13A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23998-1-AP targets ANKRD13A in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKRD13A fusion protein Ag21200
Full Name ankyrin repeat domain 13A
Calculated Molecular Weight 590 aa, 68 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC032833
Gene Symbol ANKRD13A
Gene ID (NCBI) 88455
RRIDAB_2879397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD13A, also named as ANKRD13, a 590 amino acid protein, which contains two ANK repeats and four UIM (ubiquitin-interacting motif) repeats. ANKRD13A localizes in the cell membrane. ANKRD13A as ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin, not bind 'Lys-48'-linked ubiquitin. ANKRD13A positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane.

Protocols

Product Specific Protocols
WB protocol for ANKRD13A antibody 23998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

J Biol Chem

CYYR1 promotes the degradation of the E3 ubiquitin ligase WWP1 and is associated with favorable prognosis in breast cancer

Authors - Tiphaine Perron
View Article