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  • Featured Product
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ANKRD11 Polyclonal antibody

ANKRD11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55364-1-AP

Synonyms

ANCO 1, ANCO1, ANKRD11, ankyrin repeat domain 11, LZ16, T13

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ANKRD11 Polyclonal antibody (55364-1-AP)

    WB analysis of HeLa using 55364-1-AP

    WB result of ANKRD11 antibody (55364-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANKRD11 transfected HeLa cells.

  • Western Blot (WB) analysis of BxPC-3 cells using ANKRD11 Polyclonal antibody (55364-1-AP)

    WB analysis of BxPC-3 using 55364-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ANKRD11 Polyclonal antibody (55364-1-AP)

    WB analysis of HeLa using 55364-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using ANKRD11 Polyclonal antibody (55364-1-AP)

    WB analysis of BxPC-3 using 55364-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ANKRD11 Polyclonal antibody (55364-1-AP)

    WB analysis of HeLa using 55364-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55364-1-AP targets ANKRD11 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ankyrin repeat domain 11
Calculated Molecular Weight 298 kDa
Observed Molecular Weight 150-170 kDa
GenBank Accession NumberNM_013275
Gene Symbol ANKRD11
Gene ID (NCBI) 29123
RRIDAB_11232213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD11, also named as ANCO1, may recruit HDACs to the p160 coactivators/nuclear receptor complex to inhibit ligand-dependent transactivation. This antibody is specific to ANKRD11. ANKRD11 exists 150-170 kDa isoform.

Protocols

Product Specific Protocols
WB protocol for ANKRD11 antibody 55364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Med Genet A

Two loss-of-function ANKRD11 variants in Chinese patients with short stature and a possible molecular pathway.

Authors - Tingting Zhang
View Article
mouseIHC,IF

J Physiol Biochem

The effect of aspartame on accelerating caspase-dependent apoptosis of pancreatic islet via ZIPK/STAT3/caspase 3 signaling pathway

Authors - Haiying Hu
View Article