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  • Featured Product
  • KD/KO Validated

AMFR/GP78 Polyclonal antibody

AMFR/GP78 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16675-1-AP

Synonyms

AMF receptor, isoform 2, AMFR, GP78, RING finger protein 45, RNF45

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AMFR/GP78 Polyclonal antibody (16675-1-AP)

    WB analysis of HepG2 using 16675-1-AP

    WB result of AMFR antibody (16675-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMFR transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using AMFR/GP78 Polyclonal antibody (16675-1-AP)

    WB analysis using 16675-1-AP

    HepG2 lysates were subjected to SDS PAGE followed by western blot with 16675-1-AP (AMFR antibody) at dilution of 1:4000 incubated at 37℃ and 100℃ for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using AMFR/GP78 Polyclonal antibody (16675-1-AP)

    WB analysis of MCF-7 using 16675-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16675-1-AP (AMFR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using AMFR/GP78 Polyclonal antibody (16675-1-AP)

    IP experiment of MCF-7 using 16675-1-AP

    IP result of anti-AMFR/GP78 (IP:16675-1-AP, 4ug; Detection:16675-1-AP 1:300) with MCF-7 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using AMFR/GP78 Polyclonal antibody (16675-1-AP)

    IHC staining of mouse ovary using 16675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16675-1-AP (AMFR/GP78 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using AMFR/GP78 Polyclonal antibody (16675-1-AP)

    IHC staining of mouse ovary using 16675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16675-1-AP (AMFR/GP78 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AMFR/GP78 Polyclonal antibody (16675-1-AP)

    IHC staining of human kidney using 16675-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16675-1-AP (AMFR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AMFR/GP78 Polyclonal antibody (16675-1-AP)

    IHC staining of human kidney using 16675-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16675-1-AP (AMFR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AMFR/GP78 Polyclonal antibody (16675-1-AP)

    IF Staining of HepG2 using 16675-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMFR/GP78 antibody (16675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using AMFR/GP78 Polyclonal antibody (16675-1-AP)

    FC experiment of HepG2 using 16675-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human AMFR/GP78 (16675-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected in37°C incubated HepG2 cells, HepG2 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse ovary tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16675-1-AP targets AMFR/GP78 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMFR/GP78 fusion protein Ag10178
Full Name autocrine motility factor receptor
Calculated Molecular Weight 643 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC069197
Gene Symbol AMFR/GP78
Gene ID (NCBI) 267
RRIDAB_2226463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).

Protocols

Product Specific Protocols
WB protocol for AMFR/GP78 antibody 16675-1-APDownload protocol
IHC protocol for AMFR/GP78 antibody 16675-1-APDownload protocol
IF protocol for AMFR/GP78 antibody 16675-1-APDownload protocol
IP protocol for AMFR/GP78 antibody 16675-1-APDownload protocol
FC protocol for AMFR/GP78 antibody 16675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.

Authors - Fabien Llambi
  • KD Validated
View Article
human, mouseWB, IF

Immunity

The E3 Ubiquitin Ligase AMFR and INSIG1 Bridge the Activation of TBK1 Kinase by Modifying the Adaptor STING.

Authors - Qiang Wang
  • KD Validated
View Article
humanIF

PLoS Biol

Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.

Authors - Guang Gao
View Article
mouseWB

Brief Bioinform

Standigm ASK™: knowledge graph and artificial intelligence platform applied to target discovery in idiopathic pulmonary fibrosis

Authors - Seokjin Han
View Article
humanWB

EMBO Rep

MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.

Authors - Sandra Stefanovic-Barrett
  • KO Validated
View Article
humanWB

Elife

Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.

Authors - Jianneng Li
  • KD Validated
View Article