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ALG5 Polyclonal antibody

ALG5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16046-1-AP

Synonyms

HSPC149, EC:2.4.1.117, DolP-glucosyltransferase, Dolichyl-phosphate beta-glucosyltransferase, Asparagine-linked glycosylation protein 5 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using ALG5 Polyclonal antibody (16046-1-AP)

    WB analysis of human kidney using 16046-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ALG5 Polyclonal antibody (16046-1-AP)

    WB analysis of mouse liver using 16046-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of mouse liver using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of mouse liver using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of human liver cancer using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of human liver cancer using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of human placenta using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ALG5 Polyclonal antibody (16046-1-AP)

    IHC staining of human placenta using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ALG5 Polyclonal antibody (16046-1-AP)

    IF Staining of HEK-293 using 16046-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16046-1-AP (ALG5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman kidney tissue, mouse liver tissue
Positive IHC detected inmouse liver tissue, human liver cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16046-1-AP targets ALG5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALG5 fusion protein Ag8990
Full Name asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog (S. cerevisiae)
Calculated Molecular Weight 324 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC012531
Gene Symbol ALG5
Gene ID (NCBI) 29880
RRIDAB_2273966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dolichyl-phosphate beta-glucosyltransferase(ALG5), belongs to the family of glycosyltransferases, specifically the hexosyltransferases. ALG5 is widely expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung. ALG5 can catalyze the chemical reaction between UDP-glucose and UDP with dolichyl phosphate.

Protocols

Product Specific Protocols
WB protocol for ALG5 antibody 16046-1-APDownload protocol
IHC protocol for ALG5 antibody 16046-1-APDownload protocol
IF protocol for ALG5 antibody 16046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols