• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ALDH9A1 Polyclonal antibody

ALDH9A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26621-1-AP

Synonyms

EC:1.2.1.19, ALDH9, ALDH7, ALDH4, 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of NIH/3T3 cells using ALDH9A1 Polyclonal antibody (26621-1-AP)

    WB analysis of NIH/3T3 using 26621-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ALDH9A1 Polyclonal antibody (26621-1-AP)

    WB analysis of HEK-293 using 26621-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALDH9A1 Polyclonal antibody (26621-1-AP)

    IHC staining of human liver cancer using 26621-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ALDH9A1 Polyclonal antibody (26621-1-AP)

    IHC staining of human liver cancer using 26621-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ALDH9A1 Polyclonal antibody (26621-1-AP)

    IHC staining of human kidney using 26621-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ALDH9A1 Polyclonal antibody (26621-1-AP)

    IHC staining of human kidney using 26621-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ALDH9A1 Polyclonal antibody (26621-1-AP)

    IF Staining of HeLa using 26621-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDH9A1 antibody (26621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

26621-1-AP targets ALDH9A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH9A1 fusion protein Ag24312
Full Name aldehyde dehydrogenase 9 family, member A1
Calculated Molecular Weight 518 aa, 56 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC151140
Gene Symbol ALDH9A1
Gene ID (NCBI) 223
RRIDAB_2880577
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldehyde dehydrogenase 9 family member A1(ALDH9A1), belongs to the aldehyde dehydrogenase family of proteins. ALDH9A1 has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. ALDH9A1 catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid. ALDH9A1 has 3 isoforms with the molecular mass of 46-56 kDa.

Protocols

Product Specific Protocols
WB protocol for ALDH9A1 antibody 26621-1-APDownload protocol
IHC protocol for ALDH9A1 antibody 26621-1-APDownload protocol
IF protocol for ALDH9A1 antibody 26621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Mitochondrial Protein Cox7b Is a Metabolic Sensor Driving Brain-Specific Metastasis of Human Breast Cancer Cells

Authors - Marine C N M Blackman
View Article
humanWB

Proteomes

Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.

Authors - Henrick Horita
View Article