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ALDH6A1 Polyclonal antibody

ALDH6A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20452-1-AP

Synonyms

ALDH6A1, MMSADHA, MMSDH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH6A1 Polyclonal antibody (20452-1-AP)

    WB analysis using 20452-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    WB analysis of mouse heart using 20452-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    WB analysis of mouse kidney using 20452-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALDH6A1 Polyclonal antibody (20452-1-AP)

    WB analysis using 20452-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20452-1-AP (ALDH6A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    IHC staining of human liver using 20452-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    IHC staining of human liver using 20452-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    IHC staining of human hepatocirrhosis using 20452-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    IHC staining of human hepatocirrhosis using 20452-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    IHC staining of human kidney using 20452-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    IHC staining of human kidney using 20452-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    IHC staining of human kidney using 20452-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    IHC staining of human kidney using 20452-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    IHC staining of human liver using 20452-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH6A1 Polyclonal antibody (20452-1-AP)

    IHC staining of human liver using 20452-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20452-1-AP (ALDH6A1 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inrat liver tissue, mouse heart tissue, mouse kidney tissue, HEK-293 cells, rat kidney tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue, human kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:12000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 20452-1-AP is ALDH6A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH6A1 fusion protein Ag13879
Full Name aldehyde dehydrogenase 6 family, member A1
Calculated Molecular Weight 535 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC004909
Gene Symbol ALDH6A1
Gene ID (NCBI) 4329
RRIDAB_10697664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ALDH6A1 antibody 20452-1-APDownload protocol
IHC protocol for ALDH6A1 antibody 20452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

RNA Sequencing of Collecting Duct Renal Cell Carcinoma Suggests an Interaction between miRNA and Target Genes and a Predominance of Deregulated Solute Carrier Genes.

Authors - Sven Wach
View Article
humanWB

Front Neurol

Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.

Authors - Matthias Höllerhage
View Article
humanWB

Theranostics

DNMT1 mediates the disturbed flow-induced endothelial to mesenchymal transition through disrupting β-alanine and carnosine homeostasis

Authors - Jianan Zhao
View Article