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  • Featured Product
  • KD/KO Validated

ALDH3B2 Polyclonal antibody

ALDH3B2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15746-1-AP

Synonyms

Aldehyde dehydrogenase 8, ALDH3B2, ALDH8

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using ALDH3B2 Polyclonal antibody (15746-1-AP)

    WB analysis of mouse lung using 15746-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15746-1-AP (ALDH3B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ALDH3B2 Polyclonal antibody (15746-1-AP)

    WB analysis of HepG2 using 15746-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15746-1-AP (ALDH3B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using ALDH3B2 Polyclonal antibody (15746-1-AP)

    WB analysis of BxPC-3 using 15746-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15746-1-AP (ALDH3B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human adrenal gland tissue using ALDH3B2 Polyclonal antibody (15746-1-AP)

    IHC staining of human adrenal gland using 15746-1-AP

    Immunohistochemical analysis of paraffin-embedded human adrenal gland using 15746-1-AP (ALDH3B2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human adrenal gland tissue using ALDH3B2 Polyclonal antibody (15746-1-AP)

    IHC staining of human adrenal gland using 15746-1-AP

    Immunohistochemical analysis of paraffin-embedded human adrenal gland using 15746-1-AP (ALDH3B2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ALDH3B2 Polyclonal antibody (15746-1-AP)

    IF Staining of HeLa using 15746-1-AP

    Immunofluorescent analysis of Hela cells, using ALDH3B2 antibody 15746-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse lung tissue, BxPC-3 cells, HepG2 cells
Positive IHC detected inhuman adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15746-1-AP targets ALDH3B2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH3B2 fusion protein Ag8197
Full Name aldehyde dehydrogenase 3 family, member B2
Calculated Molecular Weight 395 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC007685
Gene Symbol ALDH3B2
Gene ID (NCBI) 222
RRIDAB_2242461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH3B2(aldehyde dehydrogenase family 3 member B2) is also named as ALDH8(aldehyde dehydrogenase 8) and belongs to the aldehyde dehydrogenase family. The ALDH3B2 gene contains an in-frame stop codon at the seventeenth codon position from the first methionine,suggesting that ALDH3B2 may be a pseudogene(PMID:15237855) and encodes the human salivary gland and placental ALDH(PMID: 22206977). This protein shares 83% sequence identity with ALDH3B1(PMID:18611112 ).

Protocols

Product Specific Protocols
WB protocol for ALDH3B2 antibody 15746-1-APDownload protocol
IHC protocol for ALDH3B2 antibody 15746-1-APDownload protocol
IF protocol for ALDH3B2 antibody 15746-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

Aldehyde dehydrogenase 3B2 promotes the proliferation and invasion of cholangiocarcinoma by increasing Integrin Beta 1 expression

Authors - Yue Wang
  • KD Validated
View Article
humanWB

Authors -
View Article