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  • Featured Product
  • KD/KO Validated

ALDH2 Polyclonal antibody

ALDH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15310-1-AP

Synonyms

ALDHI, ALDH-E2, ALDHE2, ALDH E2, ALDH class 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ALDH2 Polyclonal antibody (15310-1-AP)

    WB analysis using 15310-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALDH2 Polyclonal antibody (15310-1-AP)

    WB analysis using 15310-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ALDH2 Polyclonal antibody (15310-1-AP)

    WB analysis of human heart using 15310-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP (ALDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ALDH2 Polyclonal antibody (15310-1-AP)

    IP experiment of HepG2 using 15310-1-AP

    IP result of anti-ALDH2 (IP:15310-1-AP, 4ug; Detection:15310-1-AP 1:8000) with HepG2 cells lysate 2040 ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH2 Polyclonal antibody (15310-1-AP)

    IHC staining of human liver using 15310-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP (ALDH2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH2 Polyclonal antibody (15310-1-AP)

    IHC staining of human liver using 15310-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP (ALDH2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human normal colon using ALDH2 Polyclonal antibody (15310-1-AP)

    IHC staining of human normal colon using 15310-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15310-1-AP (ALDH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using ALDH2 Polyclonal antibody (15310-1-AP)

    IHC staining of human normal colon using 15310-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15310-1-AP (ALDH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ALDH2 Polyclonal antibody (15310-1-AP)

    IF Staining of A549 using 15310-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALDH2 antibody (15310-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ALDH2 Polyclonal antibody (15310-1-AP)

    IF Staining of A549 using 15310-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ALDH2 antibody (15310-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ALDH2 Polyclonal antibody (15310-1-AP)

    IF Staining of A549 using 15310-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 15310-1-AP (ALDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, human heart tissue, mouse testis tissue, rat testis tissue, HepG2 cells, HuH-7 cells, mouse kidney tissue, mouse liver tisue, rat kidney tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15310-1-AP targets ALDH2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH2 fusion protein Ag7452
Full Name aldehyde dehydrogenase 2 family (mitochondrial)
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC002967
Gene Symbol ALDH2
Gene ID (NCBI) 217
RRIDAB_2224185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Protocols

Product Specific Protocols
WB protocol for ALDH2 antibody 15310-1-APDownload protocol
IHC protocol for ALDH2 antibody 15310-1-APDownload protocol
IF protocol for ALDH2 antibody 15310-1-APDownload protocol
IP protocol for ALDH2 antibody 15310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cancer Discov

Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia.

Authors - Zhaolin Yang
  • KO Validated
View Article
humanWB

Blood

Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.

Authors - Anfeng Mu
  • KO Validated
View Article
human,mouseWB,IHC

Mol Cell

Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans.

Authors - Felix A Dingler
  • KO Validated
View Article
mouseWB

Mol Cell

Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.

Authors - Nina Oberbeck
View Article
human,mouseWB,IP,IF

J Clin Invest

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Authors - Shanshan Zhong
  • KO Validated
View Article
human,mouseWB,IF,IP

EMBO J

p53 protects against alcoholic fatty liver disease via ALDH2 inhibition

Authors - Pengbo Yao
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Hua (Verified Customer) (03-26-2021)

Excellent antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human liver, mouse liver, mouse brain / HepG2
ALDH2 Antibody Western Blot validation (1:2000 dilution) in human liver, mouse liver, mouse brain / HepG2 (Cat no:15310-1-AP)